Successful transition from fed-batch to continuous manufacturing within a mAb process development cycle

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Factors Affecting Retention in the Philippine National Rural Physician Deployment Program From 2012 to 2019: A Mixed Methods Study

Background To address the maldistribution of healthcare providers and the shortage of physicians in geographically isolated and disadvantaged areas of the Philippines, the Philippine National Rural Physician Deployment Program, or more commonly known as the Doctors to the Barrios (DTTB) program was established in 1993. However, as of 2011, only 18% of the DTTBs chose to stay in their assigned municipalities after their two-year deployment, termed retention. This study aims to identify the individual, local, work, national, and international factors affecting the retention of DTTBs in their assigned communities after their two-year deployment. Methods A descriptive, mixed-methods, explanatory design was used. For the quantitative part, the modified and updated Stayers Questionnaire was given to all current DTTBs present in a Continuing Medical Education session in the Development Academy of the Philippines. Descriptive statistics were then presented. For the qualitative part, individual, semi-structured key informant interviews were conducted in-person or via phone with current and alumni DTTBs from 2012 to 2019. Proceedings of the interviews were transcribed, translated, and analyzed thematically. Results 102 current DTTBs participated in the quantitative part of our study, while 10 current and former DTTBs participated in the interviews. Demographic factors and location, personal beliefs, well-being, friends and family dynamics, and perceptions about work were the individual factors identified to affect retention. Social working conditions, career development, and infrastructure, medical equipment, and supplies were among the work factors identified to affect retention. Geography, living conditions, local social needs, and technology were among the local factors identified to affect retention. Compensation, the recently signed Universal Healthcare Law, and Safety and Security were identified as national factors that could affect retention. International factors did not seem to discourage DTTBs from staying in their communities. Conclusions A host of individual, work-related, local, national, and international factors influence the DTTB’s decision to be retained in different, complex, interconnected, and dynamic ways. We also identified implementation issues in the DTTB program and suggested interventions to encourage retention

The impact of sports business resilience, innovative broadcasting strategies, and co-creation strategy on consumption of sporting events with the moderating role of team performance: A case study on San Miguel Corporation

The aim of this study is to examine, explain, and describe the impact of Sports Business Resilience (SBR), Innovative Broadcasting Strategies (IBS), and Co-Creation Strategies (CCS) on Sports Consumption (SC) with the added moderating effect of Team Performance (TP) to identify the best possible courses of action to recover from the repercussions of the COVID-19 pandemic to the sports industry. To achieve this, the researchers conducted an interview on a key person in the management of the organization. Results of the study show that SBR affects the occurrence of consumption, IBS impacts accessibility and intention for consumption, and CCS influences fans’ inclination to rewatch and support future games. However, unique from the other variables, only consumption from IBS is disturbed by presence of TP. Thus, the researchers concluded that the proper course of action for SBR is the maximization of trend changes in the aspect of the 4Ps. For IBS, utilizing existing innovative technologies in enhancing the factors of content, perceived usefulness, and ease of use, and minimizing effects of TP. Lastly, for CCS, capitalizing the hedonic engagement and humanitarian interest of consumers

G Protein Mono-ubiquitination by the Rsp5 Ubiquitin Ligase*S⃞

Emerging evidence suggests that ubiquitination serves as a protein trafficking signal in addition to its well characterized role in promoting protein degradation. The yeast G protein α subunit Gpa1 represents a rare example of a protein that undergoes both mono- and poly-ubiquitination. Whereas mono-ubiquitinated Gpa1 is targeted to the vacuole, poly-ubiquitinated Gpa1 is directed instead to the proteasome. Here we investigate the structural requirements for mono- and poly-ubiquitination of Gpa1. We find that variants of Gpa1 engineered to be unstable are more likely to be poly-ubiquitinated and less likely to be mono-ubiquitinated. In addition, mutants that cannot be myristoylated are no longer mono-ubiquitinated but are still polyubiquitinated. Finally, we show that the ubiquitin ligase Rsp5 is necessary for Gpa1 mono-ubiquitination in vivo and that the purified enzyme is sufficient to catalyze Gpa1 mono-ubiquitination in vitro. Taken together, these data indicate that mono- and poly-ubiquitination have distinct enzyme and substrate recognition requirements; whereas poly-ubiquitination targets misfolded protein for degradation, a distinct ubiquitination apparatus targets the fully mature, fully myristoylated G protein for mono-ubiquitination and delivery to the vacuole

HORIZON 2020 EuPRAXIA Design Study

The Horizon 2020 Project EuPRAXIA (European Plasma Research Accelerator with eXcellence In Applications) aims at producing a design report of a highly compact and cost-effective European facility with multi-GeV electron beams using plasma as the acceleration medium. The accelerator facility will be based on a laser and/or a beam driven plasma acceleration approach and will be used for photon science, high-energy physics (HEP) detector tests, and other applications such as compact X-ray sources for medical imaging or material processing. EuPRAXIA started in November 2015 and will deliver the design report in October 2019. EuPRAXIA aims to be included on the ESFRI roadmap in 2020